Isolation of Human Umbilical Vein Endothelial Cells (HUVEC)
نویسندگان
چکیده
منابع مشابه
Isolation of human umbilical vein endothelial cells (HUVEC).
Angiogenesis is a complex multi-step process, where in response to angiogenic stimuli, new vessels are created from the existing vasculature. These steps include: degradation of the basement membrane, proliferation and migration (sprouting) of endothelial cells (EC) into the extracellular matrix, alignment of EC into cords, lumen formation, anastomosis, and formation of a new basement membrane....
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Introduction Over the last decades, considerable levels of electromagnetic fields (EMFs) have been characterized in the living environment. Recent epidemiological studies on occupational and residential exposure to EMF have shown that 50/60 Hz fields, known as extremely low frequencies (ELF), have various biological effects, such as angiogenesis. This study aimed to investigate the effect of en...
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introduction: proteolytic enzymes, specially collagenase, are used to digest extracellular matrix, cells isolation and primary culture. it is important to find new sources of plant or animal protease instead of bacterial or tissue collagenase. in the present research, actinidin, a plentiful protease in kiwifriut, was used to isolate human umbilical vein endothelial cells. materials and methods:...
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Mucin 1 (MUC1) is a membrane-bound glycoprotein that is expressed by various epithelial cell types. MUC1 functions include modulation of cell adhesion, signal transduction, lubrication and hydration of epithelial surfaces, and their protection from infection. In this study we demonstrated that MUC1 is expressed in human umbilical vein endothelial cells (HUVECs) and could be released/shed from c...
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BACKGROUND Atherosclerosis and stent re-stenosis are problems that are accompanied with high morbidity and mortality. Endothelial cell proliferation plays a role in both diseases, so the quest for potent inhibitors is still ongoing. AIM The flavonoid pinostrobin previously showed cytotoxic effects on different cell lines. In this investigation, we tested the antiproliferative effect of pinost...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2007
ISSN: 1940-087X
DOI: 10.3791/183